Enghild Lab Enghild Lab Enghild Lab Enghild Lab

Fatty Acids

Lone Hansen, Toke Peter Krogager Hansen

Dige Since Procter & Gamble introduced Crisco shortening in 1911 trans fatty acids have become an increasing component of our daily diet. The process of partial hydrogenation of edible oils leads to the formation of trans fatty from polyunsaturated cis-fatty acids (1). During the past 20 years emerging concern have been raised about the presence of trans fatty acids in food, as they have been associated with an increase in coronary heart disease (2) and myocardial infarction (3). It has been shown that with increased intake of trans fatty acids the plasma level of LDL-cholesterol goes up(4). The mechanisms behind these observations are still more or less unknown.

Our aim is to find plasma biomarkers for intake of trans fatty acids and further to understand the mechanisms behind these adverse health effects. We use human hepatoma cells, HepG2, as a model system for human metabolism and response to trans fatty acids. To investigate the secreted proteome from HepG2 cells we use Difference In Gel Electrophoresis (DIGE) and Stable Isotope Labeling with Amino acids in Cell culture (SILAC) further mRNA expression analysis is performed to unravel underlying mechanisms. More than 10 potential biomarkers has until now been found and these will be tested in blood samples from human subjects eating a diet rich in trans fatty acids. We are also investigating the effect regarding different trans fatty acids and it seems that the position of the trans double bond has a big influence on the cellular response in HepG2 cells.

SILAC & Microarray analysis

1. Scholfield, C.,R., Jones, E.,P., Butterfield, R.,O., and Dutton, H.,J. (1963) Hydrogenation of Linolenate. Fractionation of Isomeric Ester by Countercurrent Distribution with an Argentation System. 35, 386-387, 388, 389.
2. Hodgson, J. M., Wahlqvist, M. L., Boxall, J. A., and Balazs, N. D. (1996) Platelet Trans Fatty Acids in Relation to Angiographically Assessed Coronary Artery Disease. Atherosclerosis. 120, 147-154.
3. Clifton, P. M., Keogh, J. B., and Noakes, M. (2004) Trans Fatty Acids in Adipose Tissue and the Food Supply are Associated with Myocardial Infarction. J. Nutr. 134, 874-879.
4. Mensink, R. P., and Katan, M. B. (1990) Effect of Dietary Trans Fatty Acids on High-Density and Low-Density Lipoprotein Cholesterol Levels in Healthy Subjects. N. Engl. J. Med. 323, 439-445.